| 质粒名称: | HER2 WT |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 16257 |
| 存储实验室: | Mien-Chie Hung |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 9862bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HER2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4400bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | HindIII(未破坏) |
| 3’克隆位点: | HindIII (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | BGHrev |
|
文献引用方法 |
|
| 材料和方法部分: | HER2 WT was a gift from Mien-Chie Hung (Addgene plasmid # 16257 ; http://n2t.net/addgene:16257 ; RRID:Addgene_16257) |
| 参考文献部分: | Upregulation of CXCR4 is essential for HER2-mediated tumor metastasis. Li YM, Pan Y, Wei Y, Cheng X, Zhou BP, Tan M, Zhou X, Xia W, Hortobagyi GN, Yu D, Hung MC. Cancer Cell. 2004 Nov . 6(5):459-69. 10.1016/j.ccr.2004.09.027 PubMed 15542430 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS HER2 WT 9862 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS HER2 WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9862)
AUTHORS Li YM, Pan Y, Wei Y, Cheng X, Zhou BP, Tan M, Zhou X, Xia W,
Hortobagyi GN, Yu D, Hung MC
TITLE Upregulation of CXCR4 is essential for HER2-mediated tumor
metastasis.
JOURNAL Cancer Cell. 2004 Nov . 6(5):459-69.
PUBMED 15542430
REFERENCE 2 (bases 1 to 9862)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9862
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(5415..5433)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5416..5433)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5453..5470)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5459..5683
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5729..6157
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5816..5835)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6026..6047
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6166..6186)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6171..6500
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6351..6486
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6413..6432
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6567..7361
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6621..6640)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7231..7250
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7535..7656
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7572..7591)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7626..7645
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7705..7721)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7705..7721)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7718..7740)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7729..7745
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7753..7783)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7798..7819
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7936..7953)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8107..8695)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8187..8206)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8866..9726)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9489..9508
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9727..9831)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggggca
901 gccgcgcgcc ccttcccacg gggcccttta ctgcgccgcg cgcccggccc ccacccctcg
961 cagcaccccg cgccccgcgc cctcccagcc gggtccagcc ggagccatgg ggccggagcc
1021 gcagtgagca ccatggagct ggcggccttg tgccgctggg ggctcctcct cgccctcttg
1081 ccccccggag ccgcgagcac ccaagtgtgc accggcacag acatgaagct gcggctccct
1141 gccagtcccg agacccacct ggacatgctc cgccacctct accagggctg ccaggtggtg
1201 cagggaaacc tggaactcac ctacctgccc accaatgcca gcctgtcctt cctgcaggat
1261 atccaggagg tgcagggcta cgtgctcatc gctcacaacc aagtgaggca ggtcccactg
1321 cagaggctgc ggattgtgcg aggcacccag ctctttgagg acaactatgc cctggccgtg
1381 ctagacaatg gagacccgct gaacaatacc acccctgtca caggggcctc cccaggaggc
1441 ctgcgggagc tgcagcttcg aagcctcaca gagatcttga aaggaggggt cttgatccag
1501 cggaaccccc agctctgcta ccaggacacg attttgtgga aggacatctt ccacaagaac
1561 aaccagctgg ctctcacact gatagacacc aaccgctctc gggcctgcca cccctgttct
1621 ccgatgtgta agggctcccg ctgctgggga gagagttctg aggattgtca gagcctgacg
1681 cgcactgtct gtgccggtgg ctgtgcccgc tgcaaggggc cactgcccac tgactgctgc
1741 catgagcagt gtgctgccgg ctgcacgggc cccaagcact ctgactgcct ggcctgcctc
1801 cacttcaacc acagtggcat ctgtgagctg cactgcccag ccctggtcac ctacaacaca
1861 gacacgtttg agtccatgcc caatcccgag ggccggtata cattcggcgc cagctgtgtg
1921 actgcctgtc cctacaacta cctttctacg gacgtgggat cctgcaccct cgtctgcccc
1981 ctgcacaacc aagaggtgac agcagaggat ggaacacagc ggtgtgagaa gtgcagcaag
2041 ccctgtgccc gagtgtgcta tggtctgggc atggagcact tgcgagaggt gagggcagtt
2101 accagtgcca atatccagga gtttgctggc tgcaagaaga tctttgggag cctggcattt
2161 ctgccggaga gctttgatgg ggacccagcc tccaacactg ccccgctcca gccagagcag
2221 ctccaagtgt ttgagactct ggaagagatc acaggttacc tatacatctc agcatggccg
2281 gacagcctgc ctgacctcag cgtcttccag aacctgcaag taatccgggg acgaattctg
2341 cacaatggcg cctactcgct gaccctgcaa gggctgggca tcagctggct ggggctgcgc
2401 tcactgaggg aactgggcag tggactggcc ctcatccacc ataacaccca cctctgcttc
2461 gtgcacacgg tgccctggga ccagctcttt cggaacccgc accaagctct gctccacact
2521 gccaaccggc cagaggacga gtgtgtgggc gagggcctgg cctgccacca gctgtgcgcc
2581 cgagggcact gctggggtcc agggcccacc cagtgtgtca actgcagcca gttccttcgg
2641 ggccaggagt gcgtggagga atgccgagta ctgcaggggc tccccaggga gtatgtgaat
2701 gccaggcact gtttgccgtg ccaccctgag tgtcagcccc agaatggctc agtgacctgt
2761 tttggaccgg aggctgacca gtgtgtggcc tgtgcccact ataaggaccc tcccttctgc
2821 gtggcccgct gccccagcgg tgtgaaacct gacctctcct acatgcccat ctggaagttt
2881 ccagatgagg agggcgcatg ccagccttgc cccatcaact gcacccactc ctgtgtggac
2941 ctggatgaca agggctgccc cgccgagcag agagccagcc ctctgacgtc catcatctct
3001 gcggtggttg gcattctgct ggtcgtggtc ttgggggtgg tctttgggat cctcatcaag
3061 cgacggcagc agaagatccg gaagtacacg atgcggagac tgctgcagga aacggagctg
3121 gtggagccgc tgacacctag cggagcgatg cccaaccagg cgcagatgcg gatcctgaaa
3181 gagacggagc tgaggaaggt gaaggtgctt ggatctggcg cttttggcac agtctacaag
3241 ggcatctgga tccctgatgg ggagaatgtg aaaattccag tggccatcaa agtgttgagg
3301 gaaaacacat cccccaaagc caacaaagaa atcttagacg aagcatacgt gatggctggt
3361 gtgggctccc catatgtctc ccgccttctg ggcatctgcc tgacatccac ggtgcagctg
3421 gtgacacagc ttatgcccta tggctgcctc ttagaccatg tccgggaaaa ccgcggacgc
3481 ctgggctccc aggacctgct gaactggtgt atgcagattg ccaaggggat gagctacctg
3541 gaggatgtgc ggctcgtaca cagggacttg gccgctcgga acgtgctggt caagagtccc
3601 aaccatgtca aaattacaga cttcgggctg gctcggctgc tggacattga cgagacagag
3661 taccatgcag atgggggcaa ggtgcccatc aagtggatgg cgctggagtc cattctccgc
3721 cggcggttca cccaccagag tgatgtgtgg agttatggtg tgactgtgtg ggagctgatg
3781 acttttgggg ccaaacctta cgatgggatc ccagcccggg agatccctga cctgctggaa
3841 aagggggagc ggctgcccca gccccccatc tgcaccattg atgtctacat gatcatggtc
3901 aaatgttgga tgattgactc tgaatgtcgg ccaagattcc gggagttggt gtctgaattc
3961 tcccgcatgg ccagggaccc ccagcgcttt gtggtcatcc agaatgagga cttgggccca
4021 gccagtccct tggacagcac cttctaccgc tcactgctgg aggacgatga catgggggac
4081 ctggtggatg ctgaggagta tctggtaccc cagcagggct tcttctgtcc agaccctgcc
4141 ccgggcgctg ggggcatggt ccaccacagg caccgcagct catctaccag gagtggcggt
4201 ggggacctga cactagggct ggagccctct gaagaggagg cccccaggtc tccactggca
4261 ccctccgaag gggctggctc cgatgtattt gatggtgacc tgggaatggg ggcagccaag
4321 gggctgcaaa gcctccccac acatgacccc agccctctac agcggtacag tgaggacccc
4381 acagtacccc tgccctctga gactgatggc tacgttgccc ccctgacctg cagcccccag
4441 cctgaatatg tgaaccagcc agatgttcgg ccccagcccc cttcgccccg agagggccct
4501 ctgcctgctg cccgacctgc tggtgccact ctggaaaggc ccaagactct ctccccaggg
4561 aagaatgggg tcgtcaaaga cgtttttgcc tttgggggtg ccgtggagaa ccccgagtac
4621 ttgacacccc agggaggagc tgcccctcag ccccaccctc ctcctgcctt cagcccagcc
4681 ttcgacaacc tctattactg ggaccaggac ccaccagagc ggggggctcc acccagcacc
4741 ttcaaaggga cacctacggc agagaaccca gagtacctgg gtctggacgt gccagtgtga
4801 accagaaggc caagtccgca gaagccctga tgtgtcctca gggagcaggg aaggcctgac
4861 ttctgctggc atcaagaggt gggagggccc tccgaccact tccaggggaa cctgccatgc
4921 caggaacctg tcctaaggaa ccttccttcc tgcttgagtt cccagatggc tggaaggggt
4981 ccagcctcgt tggaagagga acagcactgg ggagtctttg tggattctga ggccctgccc
5041 aatgagactc tagggtccag tggatgccac agcccagctt ggccctttcc ttccagatcc
5101 tgggtactga aagccttagg gaagctggcc tgagagggga agcggcccta agggagtgtc
5161 taagaacaaa agcgacccat tcagagactg tccctgaaac ctagtactgc cccccatgag
5221 gaaggaacag caatggtgtc agtatccagg ctttgtacag agtgcttttc tgtttagttt
5281 ttactttttt tgttttgttt ttttcaagct tggtaccgag ctcggatcca ctagtaacgg
5341 ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
5401 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
5461 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
5521 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
5581 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
5641 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
5701 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
5761 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
5821 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
5881 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
5941 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
6001 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
6061 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
6121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
6181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
6421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
6481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
6541 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
6601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
6661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
6721 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
6781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
6841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
6901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
6961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
7021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
7081 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
7141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
7201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
7261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
7321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
7381 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
7441 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
7501 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
7561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
7621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
7681 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
7741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
7801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
7861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
7921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
7981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
8041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
8101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
8161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
8221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
8281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
8341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
8401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
8461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
8521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
8581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
8641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
8701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
8761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
8821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
8881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
8941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
9001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
9061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
9121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
9181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
9241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
9301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
9361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
9421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
9481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
9541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
9601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
9661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
9721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
9781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
9841 ccgaaaagtg ccacctgacg tc
//
